German Shepherd Semen

Contents
Introduction
Volume and colour of the ejaculate of German shepherd semen
PH and mass activity
Sperm concentration and sperm morphology of GSD
Test for hypoosmotic swelling (HOST)
Membrane integrity of acrosomes
Plasma seminal separation
Proper Storage and Transport of Sperm Is Crucial For AI Breeding

Introduction

German shepherd dog is a very wonderful breed. Many people like to breed this dog; therefore, it’s necessary to get it from pure breeders. Canine-semen.com sells chilled and frozen German Shepherd semen worldwide. They are pure breeders and fulfil all the protocols of semen preservation to get a healthy breed. The semen will be tested, certified, and distributed by a professional air courier company. You can also get all the procedures of AI from a famous website to update your knowledge. Nowadays, AI is becoming more popular because it controls diseases and protects German shepherd semen from contamination.
The German shepherd dog has exceptional traits. To breed this type of dog, artificial insemination is necessary. Semen deposition equipment for German shepherds consists of mostly a spermatic gun and a semen deposition-specific conduit. During semen deposition, the sperm can travel backwards, and the insemination location is shallower. In artificial canine insemination, the structure of the semen deposition equipment is the determining factor in the conception rate of the decision dog. It’s important to know all the German shepherd sperm and body condition.
Body condition score, testicular volume, and semen evaluation are the three most important aspects of Breeding Soundness Evaluation (BSE). To keep it pure, the body condition score was used to assess the general health of dogs. Testicular biometry was calculated by measuring the length and width of the testis with a vernier calliper. Digital manipulation is the most commonly used method for collecting sperm in dogs. Semen parameters such as volume, sperm concentration, motility, live and dead spermatozoa, and sperm morphology in the entire ejaculate are frequently examined to determine semen quality. The functional integrity of the sperm plasma membrane is critical for spermatozoa viability and fertilization capacity.

AVAILABLE GERMAN SHEPHERD DOG SEMEN

Volume and color of the ejaculate of German shepherd semen for AI

To collect the ejaculate, a glass funnel placed in a graduated measuring plastic tube was used, and the volume was directly measured on the graduated markings on the measuring tube. The color was observed visually. It should be clear and fully transparent.
PH and mass activity
In this process, PH paper was used to test the pH of dog sperm, which ranged from 5.0 to 8.0. The mass activity of spermatozoa was measured immediately after semen collection by analyzing a drop of semen at 100x magnification without a cover slip on a clean glass slide.

Sperm concentration and sperm morphology of GSD

Sperm concentration was measured using the hemocytometer technique and was represented as millions of spermatozoa per milliliter of sperm. The morphology of sperm was investigated using a nigrosin-eosin-stained sperm smear.
The nigrosin-eosin staining method was used to distinguish between living and dead spermatozoa.
Under 1000x magnification, 200 sperms will be visible in at least ten randomly chosen microscopic areas in each sample. The proportion of abnormal sperm with head abnormalities, proximal and distal protoplasmic droplets, a distorted tail, and the overall number of abnormal sperm were determined.

Test for hypoosmotic swelling (HOST)

A volume of 10.0 l of sperm was introduced to 1 ml of mono-distilled water and incubated at 37°C for 5 minutes to examine the sperm plasma membrane integrity. Following incubation, one drop of sperm was put on a glass slide covered with a cover slip and examined under 1000x magnification. Two hundred sperms were analyzed, and enlarged tail spermatozoa percentages were computed using the technique given.

Membrane integrity of acrosomes

Watson’s technique of studying acrosome cap integrity with Giemsa stain was used. According to Blom’s categorization, acrosome morphology was classed as entire, partial, or gone.

Plasma seminal separation

The sperm was centrifuged at 1000x g for 15 minutes at room temperature. The seminal plasma was isolated and centrifuged at 10,000x g for 10 minutes at ambient temperature before being kept at -80°c. The biochemical contents of isolated seminal plasma were estimated. The total protein, albumin, Alkaline, and acid phosphatase levels in seminal plasma were measured using a colorimetric technique.

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Proper Storage and Transport of Sperm Is Crucial For Artificial Insemination Breeding

Artificial insemination (AI) allows breeders to breed dogs that may not normally be able owing to geographic location, incompatibility of behaviour, or other considerations. Recent research investigated whether antibiotics added to commercial sperm extenders to prolong shelf life decrease bacterial development in sperm samples.
Commercial sperm enhancers are used with refrigerated and frozen sperm. Extenders are liquid media that sustain spermatozoa by supplying nutrients and a buffering capacity to compensate for temperature fluctuations during storage and transport.
Cooled sperm must be delivered and implanted in a female within 24 hours after collection to maintain viability and limit the risk of illness. In addition to typical bacterial flora from a male dog’s urethra, urine in the urethra and organisms shed from prostatic or testicular fluid due to systemic illness may contaminate semen.
Only if an antibiotic is introduced to the sperm can artificial insemination protect fetuses against illness. This research evaluated two commercial canine extenders routinely used by theriogenologists or reproductive experts. One extender includes many antibiotics, whereas the other has just one.
There is likely to be a decrease of motility in the first 24 hours.” We also showed that the presence of bacteria did not affect the motility of spermatozoa. Therefore, motility cannot be used to assess whether a sample includes bacteria.
Concerning bacterial growth, 35 percent of dogs exhibited considerable bacterial growth in their sperm, which is consistent with the literature on canine sperm. The growth of bacteria was inhibited in samples stored at refrigerated temperatures but not in all samples stored at ambient temperatures.
Notably, there was no substantial increase in any of the refrigerated samples, as per the technique for transporting chilled sperm samples. The issue was whether extenders function as we believed, demonstrating that they do.

Can artificial insemination be used for all dog breeds?

Artificial insemination is better than natural mating because there is a risk of congenital diseases or vaginal issues in natural mating.
Canine-semen.com is located in Central Europe, where German Shepherds originate and where there is an emphasis on breed standards and especially breed performance.
They are part of Europe, where most of the dogs that work in the military and police around the world come from.
They have access to the best stud dogs from dog show winners to the best service dogs.
They maintain all the qualities of semen preservation and keep it at a cool temperature to boost its efficacy.
They also use excellent extenders to compensate for its motility and make the movement of German Shepherd dogs sperm easy.
They also keep in correct temperature and speed of transport to preserve the original properties of the semen.
In fact, they are the only ones in Europe who are the best semen seller; You can purchase the semen as a whole seller and individually.

Conclusion

AI in dogs is becoming more popular , and most veterinarians are also using it; therefore, it’s important to have pure and good-quality semen. Canine-semen.com sells pure German Shepherd semen at reasonable prices in Europe and now worldwide. You can also purchase German Shepherd puppies from our other website; come and visit Puppies For Sale